Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression
doi: 10.1186/s13046-020-01556-4
Figure Lengend Snippet: Hsa_circ_0000326 acted as a sponge of miR-338-3p. ( a ) Putative targets of hsa_circ_0000326 were predicted using custom Arraystar software. ( b ) Effects of five predicted miRNAs and a negative control on the luciferase activity of hsa_circ_0000326, as determined by luciferase reporter assay. ( c ) The results of the luciferase reporter assay validated the interaction between hsa_circ_0000326 and miR-338-3p. ( d ) Colocalization between hsa_circ_0000326 and miR-338-3p was observed (arrowheads) using RNA-FISH in A549 cells. The nuclei were stained with DAPI. Scale bar = 40 μm. ( e ) RNA pulldown assay in A549 cells showed hsa_circ_0000326 could interact with miR-338-3p. The bars with error bars represent the mean ± SD from three independent experiments. * P < 0.05
Article Snippet: The relationships between circRNAs and miRNAs were predicted with custom Arraystar miRNA target prediction software based on miRanda ( http://www.microrna.org ) and TargetScan ( www.targetscan.org ).
Techniques: Software, Negative Control, Luciferase, Activity Assay, Reporter Assay, Staining